Xov xwm - Natural Vanillin Production From Renewable Resources

I. Kev Taw Qhia

Vanillin yog ib qho ntawm cov nyiam tshaj plaws thiab siv dav siv tshuaj tsw qab hauv ntiaj teb.Kev lig kev cai, nws tau raug muab rho tawm los ntawm vanilla taum, uas yog kim thiab ntsib teeb meem txog kev ruaj khov thiab cov saw hlau tsis zoo.Txawm li cas los xij, nrog rau kev nce qib hauv biotechnology, tshwj xeeb tshaj yog nyob rau hauv thaj chaw ntawm microbial biotransformation, lub sijhawm tshiab rau kev tsim cov vanillin ntuj tau tshwm sim.Kev siv cov kab mob me me rau kev hloov pauv roj ntsha ntawm cov khoom siv raw khoom tau muab txoj hauv kev lag luam zoo rau kev sib txuas ntawm vanillin.Txoj hauv kev no tsis yog tsuas yog hais txog kev txhawj xeeb txog kev ruaj ntseg tab sis kuj muaj cov kev daws teeb meem tshiab rau kev lag luam tsw.Kev tshawb fawb los ntawm SRM Lub Tsev Kawm Ntawv ntawm Kev Tshawb Fawb thiab Tshuab (SRMIST) tau muab kev tshuaj xyuas dav dav ntawm eclectic txoj hauv kev rau cov khoom siv roj ntsha ntawm vanillin thiab lawv cov ntawv thov hauv cov khoom noj khoom haus, suav nrog ntau yam txuj ci rau kev siv tshuaj lom neeg ntawm vanillin los ntawm cov substrates sib txawv thiab nws ntau yam. kev siv hauv kev lag luam zaub mov.

II.Yuav Ua Li Cas Tau Txais Ntuj Vanillin Los Ntawm Cov Khoom Siv Txuas Ntxiv

Kev siv Ferulic Acid ua Substrate

Ferulic acid, muab tau los ntawm qhov chaw xws li nplej bran thiab oat bran, nthuav qhia cov qauv zoo sib xws rau vanillin thiab ua hauj lwm raws li ib tug dav siv precursor substrate rau vanillin ntau lawm.Ntau yam kab mob xws li Pseudomonas, Aspergillus, Streptomyces, thiab fungi tau ua haujlwm rau kev tsim cov vanillin los ntawm ferulic acid.Tshwj xeeb tshaj yog, cov tsiaj xws li Amycolatopsis thiab Dawb-rot fungi tau raug txheeb xyuas tias yog cov neeg sib tw tsim cov vanillin los ntawm ferulic acid.Ntau qhov kev tshawb fawb tau tshawb fawb txog kev tsim cov vanillin los ntawm ferulic acid siv cov kab mob, cov txheej txheem enzymatic, thiab immobilized systems, qhia txog ntau yam thiab muaj peev xwm ntawm txoj hauv kev no.

Enzymatic synthesis ntawm vanillin los ntawm ferulic acid koom nrog qhov tseem ceeb enzyme feruloyl esterase, uas catalyzes hydrolysis ntawm ester daim ntawv cog lus nyob rau hauv ferulic acid, tso vanillin thiab lwm yam khoom los ntawm cov khoom.Los ntawm kev tshawb nrhiav qhov zoo tshaj plaws ntawm vanillin biosynthetic enzymes nyob rau hauv cell-free systems, cov kws tshawb fawb tau tsim ib qho kev txhim kho recombinant Escherichia coli hom muaj peev xwm hloov ferulic acid (20mM) rau hauv vanillin (15mM).Tsis tas li ntawd, kev siv microbial cell immobilization tau txais kev saib xyuas vim nws cov biocompatibility zoo heev thiab ruaj khov nyob rau hauv ntau yam mob.Cov txheej txheem tshiab immobilization rau vanillin ntau lawm los ntawm ferulic acid tau tsim, tshem tawm qhov xav tau ntawm coenzymes.Txoj hauv kev no suav nrog coenzyme-tsis ywj pheej decarboxylase thiab coenzyme-tsis ywj pheej oxygenase lub luag haujlwm rau kev hloov pauv ntawm ferulic acid rau hauv vanillin.Kev sib koom ua ke ntawm FDC thiab CSO2 ua rau kev tsim cov 2.5 mg ntawm vanillin los ntawm ferulic acid nyob rau hauv kaum lub voj voog cov tshuaj tiv thaiv, kos ib qho piv txwv ntawm vanillin ntau lawm los ntawm immobilized enzyme biotechnology.

Kev siv Eugenol/Isoeugenol ua Substrate

Eugenol thiab isoeugenol, thaum raug bioconversion, tsim vanillin thiab nws cov metabolites cuam tshuam, uas tau pom tias muaj ntau yam kev siv thiab kev lag luam tseem ceeb.Ntau qhov kev tshawb fawb tau tshawb fawb txog kev siv cov noob hloov pauv thiab cov kab mob tshwm sim los ua ke los ntawm vanillin los ntawm eugenol.Lub peev xwm rau eugenol degradation tau pom nyob rau hauv ntau yam kab mob thiab cov kab mob, suav nrog tab sis tsis txwv rau Bacillus, Pseudomonas, Aspergillus, thiab Rhodococcus, qhia lawv lub peev xwm hauv eugenol-derived vanillin ntau lawm.Kev siv eugenol oxidase (EUGO) ua ib qho enzyme rau vanillin ntau lawm hauv ib puag ncig kev lag luam tau pom muaj peev xwm tseem ceeb.EUGO nthuav tawm kev ruaj ntseg thiab kev ua haujlwm dhau ntawm pH dav dav, nrog rau cov dej soluble EUGO nce kev ua ub no thiab txo lub sijhawm cov tshuaj tiv thaiv.Ntxiv mus, kev siv immobilized EUGO tso cai rau kev rov qab los ntawm biocatalyst mus txog 18 cov tshuaj tiv thaiv kab mob, ua rau ntau tshaj 12-fold nce biocatalyst tawm los.Ib yam li ntawd, immobilized enzyme CSO2 tuaj yeem txhawb kev hloov pauv ntawm isoeugenol rau hauv vanillin yam tsis muaj kev cia siab rau coenzymes.

Lwm cov substrates

Ntxiv rau ferulic acid thiab eugenol, lwm cov tshuaj xws li vanillic acid thiab C6-C3 phenylpropanoids tau raug txheeb xyuas tias muaj peev xwm substrates rau vanillin ntau lawm.Vanillic acid, ua los ntawm cov khoom lag luam ntawm lignin degradation los yog cov khoom sib tw hauv cov txheej txheem metabolic, yog suav tias yog ib qho tseem ceeb ua ntej rau bio-based vanillin ntau lawm.Tsis tas li ntawd, muab kev nkag siab rau kev siv C6-C3 phenylpropanoids rau vanillin synthesis nthuav tawm lub sijhawm tshwj xeeb rau kev tsim kho kom ruaj khov thiab muaj txiaj ntsig tshiab.

Hauv kev xaus, kev siv cov khoom siv txuas ntxiv mus rau cov khoom siv ntuj vanillin los ntawm microbial biotransformation yog ib qho kev loj hlob hauv kev lag luam tsw.Txoj hauv kev no muaj lwm txoj hauv kev rau kev tsim cov vanillin, hais txog kev txhawj xeeb txog kev ruaj ntseg thiab txo kev cia siab ntawm cov txheej txheem rho tawm ib txwm muaj.Cov ntawv thov sib txawv thiab kev lag luam muaj txiaj ntsig ntawm vanillin thoob plaws hauv kev lag luam zaub mov qhia txog qhov tseem ceeb ntawm kev tshawb fawb txuas ntxiv thiab kev txhim kho hauv cheeb tsam no.Kev nce qib yav tom ntej hauv kev tsim khoom ntawm ntuj vanillin muaj lub peev xwm los hloov pauv kev lag luam tsw, muab cov kev hloov pauv hloov pauv hauv eco-phooj ywg rau tsw tshiab.Raws li peb txuas ntxiv siv lub peev xwm ntawm cov peev txheej txuas ntxiv mus thiab kev nce qib biotechnological, kev tsim cov vanillin ntuj los ntawm ntau lub substrates nthuav tawm txoj kev cog lus rau kev tsim kho tshiab.

III.Dab tsi yog cov txiaj ntsig ntawm kev siv cov peev txheej txuas ntxiv los tsim cov vanillin ntuj

Environmentally Friendly:Siv cov khoom siv txuas ntxiv dua tshiab xws li cov nroj tsuag thiab cov khib nyiab biomass los tsim cov vanillin tuaj yeem txo qhov kev xav tau ntawm fossil fuels, txo qhov cuam tshuam tsis zoo rau ib puag ncig, thiab txo cov pa hluav taws xob hauv tsev cog khoom.

Sustainability:Kev siv cov peev txheej txuas ntxiv mus ua kom muaj kev ruaj ntseg ntawm lub zog thiab cov khoom siv raw, pab tiv thaiv cov peev txheej ntuj thiab ua tau raws li cov kev xav tau ntawm cov tiam tom ntej.

Kev tiv thaiv Biodiversity:Los ntawm kev siv cov peev txheej txuas ntxiv mus ntxiv, cov peev txheej cog qoob loo tuaj yeem tiv thaiv, uas ua rau muaj kev tiv thaiv biodiversity thiab kev saib xyuas ntawm ecological tshuav nyiaj li cas.

Khoom zoo:Piv nrog hluavtaws vanillin, ntuj vanillin tej zaum yuav muaj ntau yam zoo nyob rau hauv aroma zoo thiab ntuj yam ntxwv, uas yuav pab txhim kho qhov zoo ntawm tsw thiab tshuaj tsw qab.

Txo kev vam khom rau fossil fuels:Kev siv cov peev txheej txuas ntxiv tuaj yeem pab txo qis kev cia siab ntawm cov fossil fuels tsawg, uas muaj txiaj ntsig zoo rau kev ruaj ntseg ntawm lub zog thiab kev sib txawv ntawm lub zog.Vam tias cov ntaub ntawv saum toj no tuaj yeem teb koj cov lus nug.Yog tias koj xav tau daim ntawv pov thawj ua lus Askiv, thov qhia rau kuv paub yog li kuv tuaj yeem muab rau koj.

IV.Xaus

Lub peev xwm ntawm kev siv cov peev txheej txuas mus ntxiv los tsim cov tshuaj vanillin los ua ib qho kev hloov pauv tau zoo thiab ib puag ncig yog qhov tseem ceeb.Txoj kev no tuav cov lus cog tseg hauv kev hais txog qhov xav tau ntau ntxiv rau ntuj vanillin thaum txo qhov kev cia siab ntawm cov txheej txheem tsim khoom hluavtaws.

Ntuj vanillin tuav txoj haujlwm tseem ceeb hauv kev lag luam tsw, muaj nuj nqis rau nws cov yam ntxwv aroma thiab dav siv los ua tus neeg sawv cev flavoring hauv ntau yam khoom.Nws yog ib qho tseem ceeb uas yuav tsum tau hais txog qhov tseem ceeb ntawm tej yam ntuj tso vanillin raws li ib tug nrhiav-tom qab muaj nyob rau hauv cov zaub mov, dej haus, thiab tshuaj tsw qab industries vim nws superior sensory profile thiab cov neeg siv nyiam rau tej yam ntuj tso flavors.

Tsis tas li ntawd, thaj chaw ntawm natural vanillin ntau lawm nthuav qhia ntau lub sijhawm rau kev tshawb fawb thiab kev loj hlob ntxiv.Qhov no suav nrog kev tshawb nrhiav cov thev naus laus zis tshiab thiab cov txheej txheem tshiab los txhim kho cov txiaj ntsig thiab kev ruaj khov ntawm kev tsim cov vanillin ntuj los ntawm cov peev txheej txuas ntxiv mus.Tsis tas li ntawd, txoj kev loj hlob ntawm scalable thiab raug nqi ntau lawm txoj kev yuav ua lub luag hauj lwm tseem ceeb nyob rau hauv kev txhawb nqa dav siv ntawm tej yam ntuj tso vanillin raws li ib tug sustainable thiab eco-friendly lwm txoj hauv kev lag luam tsw.